Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58.
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Rok publikování | 2011 |
Druh | Článek v odborném periodiku |
Časopis / Zdroj | Applied and Environmental Microbiology |
Fakulta / Pracoviště MU | |
Citace | |
Doi | http://dx.doi.org/10.1128/AEM.02109-10 |
Obor | Biochemie |
Klíčová slova | Novel Haloalkane Dehalogenase DatA ;Agrobacterium tumefaciens C58 |
Popis | Abstract: We report the biochemical characterization of a novel haloalkane dehalogenase DatA isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilising residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity towards racemic mixtures of chiral brominated alkanes and esters. |
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