Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58.
| Authors | |
|---|---|
| Year of publication | 2011 |
| Type | Article in Periodical |
| Magazine / Source | Applied and Environmental Microbiology |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1128/AEM.02109-10 |
| Field | Biochemistry |
| Keywords | Novel Haloalkane Dehalogenase DatA ;Agrobacterium tumefaciens C58 |
| Description | Abstract: We report the biochemical characterization of a novel haloalkane dehalogenase DatA isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilising residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity towards racemic mixtures of chiral brominated alkanes and esters. |
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