Screen-printed multienzyme arrays for use in amperometric batch and flow systems

• Autoři: SAPELNIKOVA Svetlana; DOCK Eva; SOLNÁ Renáta; SKLÁDAL Petr; RUZGAS Tautgirdas; EMNEUS Jenny
• Rok publikování: 2003
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Analytical and Bioanalytical Chemistry
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• Obor: Biochemie
• Klíčová slova: Screen-printed sensor; Cholinesterase; Enzyme electrode
• Popis: The screen-printed technology of electrode fabrication allows construction of amperometric devices suitable for the combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor-arrays in the batch mode as well as in the flow-injection system are discussed.

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