Screen-printed multienzyme arrays for use in amperometric batch and flow systems

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Authors	SAPELNIKOVA Svetlana DOCK Eva SOLNÁ Renáta SKLÁDAL Petr RUZGAS Tautgirdas EMNEUS Jenny
Year of publication	2003
Type	Article in Periodical
Magazine / Source	Analytical and Bioanalytical Chemistry
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	Screen-printed sensor; Cholinesterase; Enzyme electrode
Description	The screen-printed technology of electrode fabrication allows construction of amperometric devices suitable for the combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor-arrays in the batch mode as well as in the flow-injection system are discussed.
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