Complex analysis of cyclin D1 expression in mantle cell lymphoma: two cyclin D1-negative cases detected
| Autoři | |
|---|---|
| Rok publikování | 2009 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | American journal of clinical pathology : official publication the American Society of Clinical Pathologists. |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://jcp.bmj.com/content/jclinpath/62/10/948.full.pdf |
| Doi | http://dx.doi.org/10.1136/jcp.2008.063701 |
| Klíčová slova | GENE; OVEREXPRESSION; CCND2 |
| Popis | Background and Aim: The cytogenetic and diagnostic hallmark of mantle cell lymphoma (MCL) is translocation t(11; 14)(q13; q32), resulting in overexpression of cyclin D1. Cyclin D1 expression was analysed in 32 cases of MCL. Methods: The t(11; 14) translocation was detected by fluorescence in situ hybridisation, level of cyclin D1 mRNA by competitive RT-PCR, and level of cyclin D1 and D2 proteins by immunohistochemistry and/or immunoblotting. Results: In 30 cases, the presence of translocation t(11; 14), a high level of cyclin D1 mRNA, and a high level of the cyclin D1 protein were confirmed. Two cyclin D1-negative cases overexpressing cyclin D2 were detected by immunoblotting. Conclusions: There are rare cyclin D1-negative cases of MCL overexpressing cyclin D2. Anti-cyclin D1 antibodies with low specificity can bind both cyclin D1 and cyclin D2, thus providing false cyclin D1-positive signals in immunohistochemical analysis. |
| Související projekty: |