Complex analysis of cyclin D1 expression in mantle cell lymphoma: two cyclin D1-negative cases detected

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Authors	ŠTEFANČÍKOVÁ Lenka MOULIS Mojmír FABIAN P. FALKOVA I. VASOVA I. KREN L. MACAK J. ŠMARDOVÁ Jana
Year of publication	2009
Type	Article in Periodical
Magazine / Source	American journal of clinical pathology : official publication the American Society of Clinical Pathologists.
MU Faculty or unit	Faculty of Science
Citation
Web	https://jcp.bmj.com/content/jclinpath/62/10/948.full.pdf
Doi	http://dx.doi.org/10.1136/jcp.2008.063701
Keywords	GENE; OVEREXPRESSION; CCND2
Description	Background and Aim: The cytogenetic and diagnostic hallmark of mantle cell lymphoma (MCL) is translocation t(11; 14)(q13; q32), resulting in overexpression of cyclin D1. Cyclin D1 expression was analysed in 32 cases of MCL. Methods: The t(11; 14) translocation was detected by fluorescence in situ hybridisation, level of cyclin D1 mRNA by competitive RT-PCR, and level of cyclin D1 and D2 proteins by immunohistochemistry and/or immunoblotting. Results: In 30 cases, the presence of translocation t(11; 14), a high level of cyclin D1 mRNA, and a high level of the cyclin D1 protein were confirmed. Two cyclin D1-negative cases overexpressing cyclin D2 were detected by immunoblotting. Conclusions: There are rare cyclin D1-negative cases of MCL overexpressing cyclin D2. Anti-cyclin D1 antibodies with low specificity can bind both cyclin D1 and cyclin D2, thus providing false cyclin D1-positive signals in immunohistochemical analysis.
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