A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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SARANGI Prabha ALTMANNOVÁ Veronika HOLLAND Cory BARTOŠOVÁ Zdenka HAO Fanfan ANRATHER Dorothea AMMERER Gustav LEE Sang Eun KREJČÍ Lumír ZHAO Xiaolan

Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj Cell Reports
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1016/j.celrep.2014.08.054
Obor Genetika a molekulární biologie
Klíčová slova STRAND BREAK REPAIR; YEAST SCHIZOSACCHAROMYCES-POMBE; HOLLIDAY JUNCTION RESOLVASE; DNA-REPAIR; SACCHAROMYCES-CEREVISIAE; PROTEIN INTERACTIONS; RAD1-RAD10 NUCLEASE; ABASIC SITES; RECOMBINATION; ENDONUCLEASE
Popis DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes in recognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing a particular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects of Saw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.
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