A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions

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Authors

SARANGI Prabha ALTMANNOVÁ Veronika HOLLAND Cory BARTOŠOVÁ Zdenka HAO Fanfan ANRATHER Dorothea AMMERER Gustav LEE Sang Eun KREJČÍ Lumír ZHAO Xiaolan

Year of publication 2014
Type Article in Periodical
Magazine / Source Cell Reports
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1016/j.celrep.2014.08.054
Field Genetics and molecular biology
Keywords STRAND BREAK REPAIR; YEAST SCHIZOSACCHAROMYCES-POMBE; HOLLIDAY JUNCTION RESOLVASE; DNA-REPAIR; SACCHAROMYCES-CEREVISIAE; PROTEIN INTERACTIONS; RAD1-RAD10 NUCLEASE; ABASIC SITES; RECOMBINATION; ENDONUCLEASE
Description DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes in recognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing a particular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects of Saw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.
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