A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions
| Authors | |
|---|---|
| Year of publication | 2014 |
| Type | Article in Periodical |
| Magazine / Source | Cell Reports |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1016/j.celrep.2014.08.054 |
| Field | Genetics and molecular biology |
| Keywords | STRAND BREAK REPAIR; YEAST SCHIZOSACCHAROMYCES-POMBE; HOLLIDAY JUNCTION RESOLVASE; DNA-REPAIR; SACCHAROMYCES-CEREVISIAE; PROTEIN INTERACTIONS; RAD1-RAD10 NUCLEASE; ABASIC SITES; RECOMBINATION; ENDONUCLEASE |
| Description | DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes in recognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing a particular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects of Saw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions. |
| Related projects: |