Assays for determination of matrix metalloproteinases and their activity

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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KŘÍZKOVÁ Soňa ZÍTKA Ondřej MASAŘÍK Michal ADAM Vojtěch STIBOROVÁ Marie ECKSCHLAGER Tomáš CHAVIS Grace J. KIZEK René

Rok publikování 2011
Druh Článek v odborném periodiku
Časopis / Zdroj Trends in Analytical Chemistry
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1016/j.trac.2011.06.016
Obor Analytická chemie, separace
Klíčová slova Activity-based profiling; Cancer; Disease marker; Enzymatic method; Fluorimetric method; Immunochemical method; In-vivo imaging; Matrix metalloproteinase (MMP); Multiple-enzyme/multiple-reagent assay system (MEMRAS); Phage display
Popis Matrix metalloproteinases (MMPs) are involved in many physiological and pathological processes. Due to their ability to cleave and to remodel components of surrounding tissues, MMPs may affect cell migration, differentiation, growth, inflammatory processes, neovascularization, wound healing, apoptosis, the uterine cycle and many other actions within the body, including those needed for tumorigenesis and other diseases. MMPs can therefore be used as potential markers for detecting various cancers, neurodegenerative, and immune and cardiovascular diseases. Numerous MMP assays were developed for clinical and research purposes, but far more attention has been devoted to understanding their biological functions. Due to differences in methodology, results obtained in various laboratory settings are difficult to compare because of the lack of standards and analyticalmethods of validation. Limits of detection of particularmethods used for identifyingMMPs are also disputable. Enzymatic, immunochemical and fluorimetric methods are particularly suitable for clinical use. In-vivo imaging methods offer many potential advantages in cancer research and diagnostics. Other methods are subject to investigation [e.g., phage display, multiple-enzyme/multiple-reagent assay system (MEMRAS) and activity-based profiling].
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