Acetylation-dependent nuclear arrangement and recruitment of BMI1 protein to UV-damaged chromatin

• Autoři: ŠUSTÁČKOVÁ Gabriela; KOZUBEK Stanislav; STIXOVÁ Lenka; LEGARTOVÁ Soňa; MATULA Pavel; ORLOVA Darya Yurevna; BÁRTOVÁ Eva
• Rok publikování: 2012
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Journal of cellular physiology
• Fakulta / Pracoviště MU: Fakulta informatiky
• www: http://onlinelibrary.wiley.com/doi/10.1002/jcp.22912/suppinfo
• Doi: http://dx.doi.org/10.1002/jcp.22912
• Obor: Genetika a molekulární biologie
• Klíčová slova: Polycomb body BMI1 protein arrangement confocal microscopy
• Popis: Polycomb group (PcG) proteins, organized into Polycomb bodies, are important regulatory components of epigenetic processes involved in the heritable transcriptional repression of target genes. Here, we asked whether acetylation can influence the nuclear arrangement and function of the BMI1 protein, a core component of the Polycomb group complex, PRC1. We used time-lapse confocal microscopy, micro-irradiation by UV laser (355 nm) and GFP technology to study the dynamics and function of the BMI1 protein. Our data indicate that the dynamics of recognition of UV-damaged chromatin, and the nuclear arrangement of BMI1 protein can be influenced by acetylation and occur as an early event prior to the recruitment of HPb to UV-irradiated chromatin.

Související projekty

• Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii