Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity

• Autoři: BENEŠ Petr; JANČEKOVÁ Blanka; KNOPFOVÁ Lucia; ŠMARDA Jan
• Rok publikování: 2011
• Druh: Konferenční abstrakty
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• Popis: The lysosomal aspartic protease cathepsin D (cath-D) is often over-expressed in breast cancer and acts as a mitogen on both cancer and stromal cells. The ability of cath-D to stimulate cancer cell migration remains controversial. To determine whether cath-D expression modulates breast cancer cell migration in vitro, we performed a real-time analysis of MDA-MB-231 breast cancer cell migration using novel methodology based on impedance measurement that enables continuous monitoring of the transition of the cells through a microporous membrane [xCELLigence equipped with CIM-plate 16 (Roche)]. We observed that altering cath-D level by either siRNA or cDNA tranfections significantly affected migration of MDA-MB-231 cells. Tumor cells have often impaired classical caspase-dependent apoptosis pathway and may be therefore more sensitive to agents that trigger alternative cell death pathways. Targeting lysosomes represents a method of choice as many human tumors have increased levels of lysosomal proteases.

Související projekty

• Molekulární podstata buněčných a tkáňových regulací
• Molekulární mechanismy proliferace a diferenciace buněk
• Mechanismus účinku prokatepsinu D na buňky rakoviny prsu
• Úloha proteinů c-Myb a Cox-2 při tvorbě střevních nádorů