Assessment of adenosine triphosphate-binding cassette subfamily B member 1 (ABCB1) mRNA expression in patients with de novo chronic myelogenous leukemia: the role of different cell types.

• Autoři: RÁČIL Zdeněk; RÁZGA Filip; MACHOVÁ POLÁKOVÁ Kateřina; BUREŠOVÁ Lucie; POLÍVKOVÁ Václava; DVOŘÁKOVÁ Dana; ŽÁČKOVÁ Daniela; KLAMOVÁ Hana; CETKOVSKÝ Petr; MAYER Jiří
• Rok publikování: 2011
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Leukemia & Lymphoma
• Fakulta / Pracoviště MU: Středoevropský technologický institut
• Doi: http://dx.doi.org/10.3109/10428194.2010.533220
• Obor: Onkologie a hematologie
• Klíčová slova: mRNA exprese; CML
• Popis: In this study, we investigated differences in ABCB1 mRNA expression measured in peripheral blood (PB) leukocytes (LEU) as well as polymorphonuclear (PMNCs) and mononuclear cells (MNCs) of PB LEU obtained from healthy volunteers and patients with de novo CML. In addition, we analyzed the relationship between the percentage of individual cells that comprise the total LEU count and ABCB1 mRNA expression assessed from the total LEU. We have shown that ABCB1 mRNA transcript levels are significantly higher in PB MNCs than in PB PMNCs and are lower in patients with de novo CML compared to healthy individuals. Moreover, we have demonstrated the importance of the cell composition of analyzed samples in ABCB1 mRNA expression analysis.

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