Quantification of Fluorescent Spots in Time Series of 3D Confocal Microscopy Images of Endoplasmic Reticulum Exit Sites Based on the HMAX Transform

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Fakultu informatiky. Oficiální stránka publikace je na webu muni.cz.
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MATULA Petr VERISSIMO Fatima WÖRZ Stefan EILS Roland PEPPERKOK Rainer ROHR Karl

Rok publikování 2010
Druh Článek ve sborníku
Konference Proceedings of SPIE Medical Imaging
Fakulta / Pracoviště MU

Fakulta informatiky

Citace
www http://spie.org/x648.html?product_id=844441
Obor Informatika
Klíčová slova Fluorescence microscopy; spot detection; HMAX transform; endoplasmic reticulum exit sites
Popis We present an approach for the quantification of fluorescent spots in time series of 3-D confocal microscopy images of endoplasmic reticulum exit sites of dividing cells. Fluorescent spots are detected based on extracted image regions of highest response using the HMAX transform and prior convolution of the 3-D images with a Gaussian kernel. The sensitivity of the involved parameters was studied and a quantitative evaluation using both 3-D synthetic and 3-D real data was performed. The approach was successfully applied to more than one thousand 3-D confocal microscopy images.
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