Subcellular Localization and In Vivo Interactions of the Arabidopsis thaliana Ethylene Receptor Family Members
| Autoři | |
|---|---|
| Rok publikování | 2008 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Molecular Plant |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://mplant.oxfordjournals.org/cgi/content/full/1/2/308 |
| Obor | Genetika a molekulární biologie |
| Klíčová slova | ethylene; ethylene receptors; signal transduction; oligomerization; protein-protein interaction; MeRA; mbSUS; Arabidopsis |
| Popis | The gaseous phytohormone ethylene regulates many developmental processes and responses to environmental conditions in higher plants. In Arabidopsis thaliana, ethylene perception and initiation of signaling are mediated by a family of five receptors which are related to prokaryotic two-component sensor histidine kinases. The transient expression of fluorescence-tagged receptors demonstrated that all ethylene receptors are targeted to the ER endomembrane network and do not localize to the plasmalemma. In support of in planta overlay studies, the ethylene receptors form homomeric and heteromeric protein complexes at the ER in living plant cells, as shown by membrane recruitment assays. A comparable in vivo interaction pattern was found in the yeast mating-based split-ubiquitin system. The overlapping but distinct expression pattern of the ethylene receptor genes suggests a differential composition of the ethylene receptor complexes in different plant tissues. |
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