A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples
| Název česky | Rychlý test na druhovou identifikaci dvou kryptických netopýrů Pipistrellus pipistrellus a P. pygmaeus na základě PCR metody a jeho využití na muzejních vzorcích a vzorcích trusu |
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| Autoři | |
| Rok publikování | 2007 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Acta Chiropterologica |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Zoologie |
| Klíčová slova | Chiroptera; cytochrome b; mtDNA; sibling species; non-invasive sampling |
| Popis | The identification of two cryptic bat species of the genus Pipistrellus using a non-destructive and quick method of multiplex PCR and agarose gel electrophoresis is described. Two primer combinations were able to produce species-specific bands that identified reliably individuals that were previously identified by mtDNA sequencing. Robustness of the method was subsequently successfully tested on 16 randomly selected free-living animals from central Europe (tissue samples obtained from a 3 mm punch of wing-membrane) identified to species on the basis of echolocation calls. Nine out of 15 museum specimens and 100% of fresh faecal samples from seven individuals were also successfully identified by this method. The described method thus provides a good way to routinely distinguish two Pipistrellus species by using non-destructive sampling of living individuals or droppings, and will be used in field studies of their ecology. |
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