Role of SdiA in Salmonella enterica serovar typhimurium physiology and virulence

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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VOLF Jiří ŠEVČÍK Mojmír HAVLÍČKOVÁ Hana ŠIŠÁK František DAMBORSKÝ Jiří RYCHLÍK Ivan

Rok publikování 2002
Druh Článek v odborném periodiku
Časopis / Zdroj Archives in Microbiology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://ncbr.chemi.muni.cz/~jiri/ABSTRACTS/archmicro02a.html
Obor Mikrobiologie, virologie
Klíčová slova VIRULENCE; TRANSCRIPTION; SALMONELLA; SDI GENE; MUTANT
Popis sdiA in Salmonella enterica serovar Typhimurium encodes a protein belonging to the LuxR family of transcriptional regulators. Initial computer analysis revealed the presence of a fur box 19 bp upstream of the start codon of the sdiA gene and a helix-turn-helix motif in the carboxy-terminal part of the SdiA protein typical for transcriptional regulators. Deletion of the fur box resulted in twofold increase of sdiA transcription. Furthermore, addition of dipyridyl, an iron chelator, to culture media increased sdiA transcription to the level observed in the fur box mutant, confirming that sdiA is suppressed in the presence of iron. When S. enterica sv. Typhimurium was grown in conditioned medium, sdiA transcription was repressed to 30% of its transcription in cells grown in fresh LB broth and this repression was independent of the fur box. Oral infection of mice with the strain lacking the helix-turn-helix domain of SdiA indicated increased virulence of such S. enterica sv. Typhimurium mutant. sdiA, dually controlled by iron concentration and culture density derived signals, may therefore play an important role in S. enterica sv. Typhimurium virulence regulation.
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