Phenotype and oxidative burst of low-density neutrophil subpopulations are altered in common variable immunodeficiency patients

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
Název česky Fenotyp a oxidační vzplanutí subpopulací neutrofilů s nízkou hustotou jsou změněny u pacientů s běžnou variabilní imunodeficiencí
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SLANINA Peter ŠTÍCHOVÁ Julie BOSÁKOVÁ Veronika STANICZKOVÁ ZAMBO Iva HORTOVÁ KOHOUTKOVÁ Marcela LÁZNIČKOVÁ Petra CHOVANCOVÁ Zita LITZMAN Jiří STEJSKALOVÁ Terezie FRIČ Jan VLKOVÁ Marcela

Rok publikování 2024
Druh Článek v odborném periodiku
Časopis / Zdroj CYTOMETRY PART B-CLINICAL CYTOMETRY
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://onlinelibrary.wiley.com/doi/10.1002/cyto.b.22150
Doi http://dx.doi.org/10.1002/cyto.b.22150
Klíčová slova common variable immunodeficiency; intravenous immunoglobulins; low-density neutrophils; oxidative burst; suppression.
Přiložené soubory
Popis Common variable immunodeficiency disorder (CVID) is the most common form of primary antibody immunodeficiency. Due to low antibody levels, CVID patients receive intravenous or subcutaneous immunoglobulin replacement therapy as treatment. CVID is associated with the chronic activation of granulocytes, including an increased percentage of low-density neutrophils (LDNs). In this study, we examined changes in the percentage of LDNs and the expression of their surface markers in 25 patients with CVID and 27 healthy donors (HD) after in vitro stimulation of whole blood using IVIg. An oxidative burst assay was used to assess the functionality of LDNs. CVID patients had increased both relative and absolute LDN counts with a higher proportion of mLDNs compared to iLDNs, distinguished based on the expression of CD10 and CD16. Immature LDNs in the CVID and HD groups had significantly reduced oxidative burst capacity compared to mature LDNs. Interestingly we observed reduced oxidative burst capacity, reduced expression of CD10 after stimulation of WB, and higher expression of PD-L1 in mature LDNs in CVID patients compared to HD cells. Our data indicate that that the functional characteristics of LDNs are closely linked to their developmental stage. The observed reduction in oxidative burst capacity in mLDNs in CVID patients could contribute to an increased susceptibility to recurrent bacterial infections among CVID patients.
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