TOOLS TO STUDY DOSAGE COMPENSATION AND PARENTAL IMPRINTING IN SILENE LATIFOLIA
| Autoři | |
|---|---|
| Rok publikování | 2023 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Dosage compensatory mechanism (DC) refers to the equalization of X-linked gene products between males and females. In old sex chromosomes systems, DC usually affects the entire X chromosomes with only few genes capable of inactivation escape. Nevertheless, in species with evolutionary young sex chromosomes, DC exhibits rather local or incomplete X chromosome inactivation, proceeding gene-by-gene or by whole locus deregulation. Despite the evidence given for the main animal species, the mechanisms through X chromosome inactivation evolves remain still enigmatic. To study early stages of DC and expression balance between sexes, we use combination of cytogenetic and NGS techniques in a species with young sex chromosome system, Silene latifolia. Using X chromosome specific marker and EdU that is incorporated into newly synthesized DNA strands, we distinguished paternal and maternal Xs. We show that parental X chromosome in females is late replicated, supported by previous evidence of transcriptome profiling and localization of active histone marks. Interestingly, only a chromosomal arm carrying PAR shows replication delay that suggests locus specific incomplete DC. Further, we ask weather polyploidy may disturb dosage compensation pattern in this model plant. To answer this question, we developed a set of tetraploid and triploid individuals with different sex chromosome constitution. This will help us to understand how and what (epi)genetic processes take a role in DC evolution, and help us to shed more light on the most fundamental question of sex chromosome biology. |
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