Vitronectin is a xeno-free and defined surface for the derivation of human embryonic stem cells
| Autoři | |
|---|---|
| Rok publikování | 2023 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Human embryonic stem cells (hESCs) have unique abilities that enable their use in cell therapy, diseasemodelling, and drug development. Their derivation is usually performed using a feeder layer, which isundefined and potentially contagious, and because of these difficulties, there is a tendency to replacefeeders with xeno-free defined substrates in recent years. We used truncated vitronectin for thederivation of hESCs for the first time, derived three hESC lines, and confirmed their undifferentiatedstate, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers. These results support the conclusion that truncated vitronectin is a defined xeno-freesubstrate that is suitable for the derivation of hESCs. |
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