Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds
| Autoři | |
|---|---|
| Rok publikování | 2023 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Electrophoresis |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/elps.202100379 |
| Doi | http://dx.doi.org/10.1002/elps.202100379 |
| Klíčová slova | bioanalysis; hemoglobin; mass spectrometry; microfluidics; single-cell analysis |
| Popis | Analysis of cellular composition and metabolism at a single-cell resolution allows gaining more information about complex relationships of cells within tissues or whole living organisms by resolving the variance stemming from the cellular heterogeneity. Mass spectrometry (MS) is a perfect analytical tool satisfying the demanding requirements of detecting and identifying compounds present in such ultralow-volume samples of high chemical complexity. However, the method of sampling and sample ionization is crucial in obtaining relevant information. In this work, we present a microfluidic sampling platform that integrates single-cell extraction from MS-incompatible media with electrical cell lysis and nanoESI-MS analysis of human erythrocytes. Hemoglobin alpha and beta chains (300 amol/cell) were successfully identified in mass spectra of single-erythrocyte lysates. |
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