Epitachophoretic preconcentration of proteins
| Autoři | |
|---|---|
| Rok publikování | 2021 |
| Druh | Článek ve sborníku |
| Konference | Proceedings of the 17th International Students Conference “Modern Analytical Chemistry” |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://web.natur.cuni.cz/analchem/isc/isc17.pdf |
| Klíčová slova | cleaning; epitachophoresis; isotachophoresis; preparative; concentration |
| Popis | This project is focused on the epitachophoretic preconcentration of Proteins from biological fluids. In this paper, the method for preparative concentration of three standards of proteins, cytochrome c, myoglobin, and hemoglobin, was developer and described. Concentration conditions, such as concentration and pH of leading and trailing electrolytes, gel composition, and time of concentration, were optimized. The recovery of each protein was at least 70% and concentration factor 50. |
| Související projekty: |