A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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CAPITAO C. TANASA Sorin FULNEČEK Jaroslav RAXWAL Vivek Kumar AKIMCHEVA S. BULANKOVA P. MIKULKOVÁ Pavlína CRHÁK KHAITOVÁ Lucie KALIDASS M. LERMONTOVA I. SCHEIDID M. ŘÍHA Karel

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj PLoS Genetics
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1009779
Doi http://dx.doi.org/10.1371/journal.pgen.1009779
Klíčová slova CHROMOSOME SEGREGATION; MITOTIC EXIT; MEIOSIS-I; GENE; PROGRESSION; HOMOLOG; SMG7; SPOROGENESIS; INITIATION; EVOLUTION
Popis Meiosis in angiosperm plants is followed by mitotic divisions to form multicellular haploid gametophytes. Termination of meiosis and transition to gametophytic development is, in Arabidopsis, governed by a dedicated mechanism that involves SMG7 and TDM1 proteins. Mutants carrying the smg7-6 allele are semi-fertile due to reduced pollen production. We found that instead of forming tetrads, smg7-6 pollen mother cells undergo multiple rounds of chromosome condensation and spindle assembly at the end of meiosis, resembling aberrant attempts to undergo additional meiotic divisions. A suppressor screen uncovered a mutation in centromeric histone H3 (CENH3) that increased fertility and promoted meiotic exit in smg7-6 plants. The mutation led to inefficient splicing of the CENH3 mRNA and a substantial decrease of CENH3, resulting in smaller centromeres. The reduced level of CENH3 delayed formation of the mitotic spindle but did not have an apparent effect on plant growth and development. We suggest that impaired spindle re-assembly at the end of meiosis limits aberrant divisions in smg7-6 plants and promotes formation of tetrads and viable pollen. Furthermore, the mutant with reduced level of CENH3 was very inefficient haploid inducer indicating that differences in centromere size is not the key determinant of centromere-mediated genome elimination.
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