Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls

Varování

Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	RAŠKA Jan KLÍMOVÁ Hana SHEARDOVÁ Kateřina FEDOROVÁ Veronika HŘÍBKOVÁ Hana POSPÍŠILOVÁ Veronika VOCHYÁNOVÁ Simona VÁŇOVÁ Tereza BOHAČIAKOVÁ Dáša
Rok publikování	2021
Druh	Článek v odborném periodiku
Časopis / Zdroj	Stem cell research
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
www	https://www.sciencedirect.com/science/article/pii/S1873506121002245?via%3Dihub
Doi	http://dx.doi.org/10.1016/j.scr.2021.102378
Klíčová slova	human iPSC lines; spontaneous late-onset Alzheimer’s disease
Popis	Human induced pluripotent stem cell (iPSC) lines were generated from patients with spontaneous late-onset Alzheimer's disease (AD) and three healthy control individuals. Peripheral blood mononuclear cells were reprogrammed with Yamanaka factors (OSKM) using a commercially available Epi5 Reprogramming Kit. The pluripotency of iPSCs was confirmed by the expression of pluripotency factors and by their ability to differentiate to all three germ layers in vitro. Newly derived cell lines can be used to model Alzheimer's disease in vitro.
Související projekty:	Klinicky relevantní biochemické, imunologické a buněčné biomarkery Alzheimerovy nemoci a stárnutí Career Restart - Stem Cell-Based Models of Neurodegeneration