12-O-Tetradecanoylphor bol-13-acetate increases cardiomyogenesis through PKC/ERK signaling

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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RADASZKIEWICZ Katarzyna Anna BECKEROVÁ Deborah WOLOSZCZUKOVÁ Lucie RADASZKIEWICZ Tomasz Witold LESÁKOVÁ Petra VONDÁLOVÁ BLANÁŘOVÁ Olga KUBALA Lukáš HUMPOLÍČEK Petr PACHERNÍK Jiří

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj Scientific reports
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://doi.org/10.1038/s41598-020-73074-4
Doi http://dx.doi.org/10.1038/s41598-020-73074-4
Klíčová slova Cell biology; Developmental biology; Stem cells
Popis 12-O-Tetradecanoylphorbol-13-acetate (TPA) is the most widely used diacylglycerol (DAG) mimetic agent and inducer of protein kinase C (PKC)-mediated cellular response in biomedical studies. TPA has been proposed as a pluripotent cell differentiation factor, but results obtained have been inconsistent. In the present study we show that TPA can be applied as a cardiomyogenesis-promoting factor for the differentiation of mouse embryonic stem (mES) cells in vitro. The mechanism of TPA action is mediated by the induction of extracellular signal-regulated kinase (ERK) activity and the subsequent phosphorylation of GATA4 transcription factor. Interestingly, general mitogens (FGF, EGF, VEGF and serum) or canonical WNT signalling did not mimic the effect of TPA. Moreover, on the basis of our results, we postulate that a TPA-sensitive population of cardiac progenitor cells exists at a certain time point (after days 6-8 of the differentiation protocol) and that the proposed treatment can be used to increase the multiplication of ES cell-derived cardiomyocytes.
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