Effect of eicosanoid biosynthesis inhibitors on the haemolymph protein profile of Galleria mellonella (Linnaeus, 1758) larvae (Lepidoptera: Pyralidae)

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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HYRŠL Pavel BUYUKGUZEL Ender BUYUKGUZEL Kemal

Rok publikování 2011
Druh Článek v odborném periodiku
Časopis / Zdroj TURKIYE ENTOMOLOJI DERGISI-TURKISH JOURNAL OF ENTOMOLOGY
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://hdl.handle.net/20.500.12628/748
Klíčová slova Eicosanoids; esculetin; Galleria mellonella; haemolymph; protein profile
Popis Eicosanoids mediate insects cellular and humoral immune reactions and stress responses. Function of these mediators can be specifically blocked using different eicosanoid biosynthesis inhibitors (EBIs). Effects of EBIs on total haemolymph protein composition have not been extensively studied in insects. We posed the hypothesis that eicosanoids also mediate physiological homeostasis by regulating protein profiles involved in stress response and other defensive reactions. To test this idea, we reared greater wax moth Galleria mellonella (Linnaeus, 1758) (Lepidoptera: Pyralidae) larvae on artificial diets containing 0.001, 0.01, 0.1 or 1.0% of specific EBIs with different mode of action: Esculetin, dexamethasone and phenidone. Feeding larvae with esculetin caused significantly dose-dependent changes in 45 kDa protein fraction (one of 16 proteins detected) using sodium dodecyl-sulphate polyacrylamide gradient gel electrophoresis followed by silver staining. Other main haemolymph proteins, lipophorins (ApoLP-I) and storage proteins, were not affected by EBIs treatments. Dexamethasone and phenidone caused no significant differences in detected protein fractions. We infer from these findings that eicosanoids, at least lipoxygenase products, have been implicated in the protein composition of insect tissues as structural and functional concept. Although it has not yet been possible to use directly EBIs for insect pest control, our results bring new data to understand physiological signaling systems in insects.
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