Rapid separation and identification of the subtypes of swine and equine influenza A viruses by electromigration techniques with UV and fluorometric detection

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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HORKA Marie KUBICEK Oldrich KUBESOVÁ Anna ROSENBERGOVA Katerina KUBICKOVA Zuzana SLAIS Karel

Rok publikování 2011
Druh Článek v odborném periodiku
Časopis / Zdroj Analyst
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://doi.org/10.1039/C0AN00896F
Doi http://dx.doi.org/10.1039/c0an00896f
Klíčová slova CAPILLARY-ELECTROPHORESIS; BIOLOGICAL PARTICLES; ZONE-ELECTROPHORESIS; ISOELECTRIC POINT; PROTEINS; MICROORGANISMS; BACTERIA; PI; MOLECULES; EVOLUTION
Popis Influenza A is viral disease, which is a cause of yearly epidemics and, potentially, pandemics. The conventional techniques used today are equipment-demanding, time-consuming and laborious. Recently, we have confirmed that the capillary isoelectric focusing is a suitable fast alternative for the verifying of virus purity. In the wide pH gradient of pH range 2.0-7.5 the isoelectric points for subtypes of equine (H3N8) and swine (H1N2) influenza A viruses were determined approximately as 6.6 and 6.5, respectively. In this contribution we have verified these findings using different isolates of different viral subtypes of swine influenza, H1N1, H1N2, and of equine influenza, H3N8, H7N7, which were separated by capillary zone electrophoresis (CZE) and capillary isoelectric focusing (CIEF) in the narrow pH gradient pH range from 6.0 to 7.0. It was found that the isoelectric points of different isolates and subtypes of equine and swine influenza are almost independent of their origin. The electromigration velocities of subtypes of equine or swine influenza viruses were dependent on the antigenic subtypes of their surface glycoproteins. The detection sensitivity of the influenza viruses labeled by the fluorescent non-ionogenic tenside based on poly(ethylene glycol)pyrenebutanoate for fluorometric detection was increased and down to ten labeled viruses were detected. The isoelectric points of the native and labeled equine and swine influenza A viruses and their subtypes do not differ. According to our experiments these methods appear to be useful for the fast preliminary differentiation of influenza viruses in future.
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