Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry

• Autoři: TVRDOŇOVÁ Michaela; VLČNOVSKÁ Marcela; VANICKOVA Lucie; KANICKÝ Viktor; ADAM Vojtěch; ASCHER Lena; JAKUBOWSKI Norbert; VACULOVIČOVÁ Markéta; VACULOVIČ Tomáš
• Rok publikování: 2019
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Analytical and Bioanalytical chemistry
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• www: http://dx.doi.org/10.1007/s00216-018-1300-7
• Doi: http://dx.doi.org/10.1007/s00216-018-1300-7
• Klíčová slova: Dot blot; Antibody labelling; Gold nanoparticles; LA-ICP-MS; Immunochemistry
• Popis: In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.

Související projekty

• CEITEC 2020
• Využití vícenásobného značení pomocí kovových nanočástic pro bio-zobrazování