Digital camera-based lipase biosensor for the determination of paraoxon

Varování

Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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POHANKA Miroslav ZAKOVA Jitka SEDLÁČEK Ivo

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj SENSORS AND ACTUATORS B: CHEMICAL
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://dx.doi.org/10.1016/j.snb.2018.06.084
Doi http://dx.doi.org/10.1016/j.snb.2018.06.084
Klíčová slova Biosensor; Liopase; Fatty acid; Inhibition
Popis This work is focused on construction of a biosensor containing unique bacterial homogenate with high lipase activity and colorimetric type of assay where camera of a smartphone was chosen as a detector. The biosensor was constructed as a tool of lipase inhibitors and paraoxon served as a representative analyte inhibiting the lipase. Psychrophilic strains of bacteria isolated from in Antarctica were tested and the best isolate P4368 having huge lipase activity was chosen. Tween assay was performed as a standard method for lipase activity determination and indoxylacetate served as a substrate of lipase measurable by smartphone camera and R, G and B color channels digital analysis. Bacteria were homogenized and immobilized on polyvinylidene difluoride membrane and indoxylacetate was immobilized in the proximity of the homogenate. Paraoxon ethyl was analyzed by the standard method and by the camera-based biosensor. The biosensor-based assay was found to determine paraoxon with limit of detection 3.72×10 -8exp mol/l and IC50 value for paraoxon was 4.00×10 -6exp mol/l. A volume 5 microliter of sample was sufficient for the assay and the sample was applied directly without any processing. In a conclusion, a simple colorimetric biosensor for the determination of venomous compounds like organophosphorus pesticides and nerve agents was constructed and promising analytical parameters were received during its characterization.
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