AFM Monitoring the Influence o f Selected Cryoprotectants on Regeneration of Cryopreserved Cells Mechanical Properties

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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GOLAN Martin JELÍNKOVÁ Šárka KRATOCHVÍLOVÁ Irena SKLÁDAL Petr PEŠL Martin ROTREKL Vladimír PŘIBYL Jan

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj Frontiers in Physiology
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://www.frontiersin.org/articles/10.3389/fphys.2018.00804/full
Doi http://dx.doi.org/10.3389/fphys.2018.00804
Klíčová slova cryopreservation; cell stiffness; AFM; fluorescence microscopy; DMSO; PEG
Popis Cryopreservation of cells (mouse embryonic fibroblasts) is a fundamental task for wide range of applications. In practice, cells are protected against damage during freezing by applications of specific cryoprotectants and freezing/melting protocols. In this study by using AFM and fluorescence microscopy we showed how selected cryoprotectants (dimethyl sulfoxide and polyethylene glycol) affected the cryopreserved cells mechanical properties (stiffness) and how these parameters are correlated with cytoskeleton damage and reconstruction. We showed how cryopreserved (frozen and thawed) cells' stiffness change according to type of applied cryoprotectant and its functionality in extracellular or intracellular space. We showed that AFM can be used as technique for investigation of cryopreserved cells surfaces state and development ex vivo. Our results offer a new perspective on the monitoring and characterization of frozen cells recovery by measuring changes in elastic properties by nanoindentation technique. This may lead to a new and detailed way of investigating the post-thaw development of cryopreserved cells which allows to distinguish between different cell parts.
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