The CD8+cell non-cytotoxic antiviral response affects RNA polymerase II-mediated human immunodeficiency virus transcription in infected CD4+cells

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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BLAŽEK Dalibor TEQUE Fernando MACKEWICZ Carl PETERLIN Matija LEVY Jay A.

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of General Virology
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000326
Doi http://dx.doi.org/10.1099/jgv.0.000326
Obor Mikrobiologie, virologie
Klíčová slova ANTI-HIV RESPONSE; CD8(+) T-CELLS; P-TEFB; FACTOR CAF; REPLICATION; INHIBITION; PATHOGENESIS; LYMPHOCYTES; ELONGATION; EXPRESSION
Popis A CD8+ cell non-cytotoxic antiviral response (CNAR), mediated by a CD8+ cell antiviral factor (CAF), is associated with a long-term healthy state in human immunodeficiency virus (HIV) infection. CNAR/CAF reduces viral transcription without a known effect on specific viral sequences in the HIV genome. In studies to define the mechanism involved in the block in viral transcription, we now report that transcription from the HIV-LTR reporter is reduced in infected CD4+ cells upon treatment with CAF. In agreement with this observation, the amount of RNA polymerase II (RNAPII) on the HIV promoter and other viral regions was strongly diminished in HIV-infected CD4+ cells co-cultivated with CNAR-expressing CD8+ cells. These results demonstrate further that CNAR/CAF has a specific role in regulating HIV transcription and a step during the preinitiation complex assembly appears to be sensitive to CNAR/CAF.
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