MicroScale thermophoretic investigation of deferiprone interaction with selected biometals
| Autoři | |
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| Rok publikování | 2016 |
| Druh | Článek ve sborníku |
| Konference | Annual Meeting of the German Pharmaceutical Society, DPhG 2016 |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Biochemie |
| Popis | MicroScale Thermophoresis (MST) method has been developed and applied for investigating the interaction of deferiprone (iron-chelator) with different biometals.The experiments were performed on Monolith NT. LabelFree MicroScale Thermophoresis system. Pretest scanning indicated good fluorescence intensity of deferiprone proper for label free experiments. Different concentrations of the intended metals in the range of 0.048 - 500.0 µM were titrated against 100 µM fixed concentration of deferiprone dissolved in 0.1 M Tris buffer at pH 7.4. The results indicated significant interactions of deferiprone with Cu2+, Zn2+, Ni2+, Co2+ and Fe3+. The data fitted to Hill equation indicates more than 1:1 stoichiometry. No binding of deferiprone with Mg2+, Ca2+ and Mn2+ were observed. The technique shows a fast and simple approach to study the binding of deferiprone to different biometals. |
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