Transgelin is upregulated in stromal cells of lymph node positive breast cancer
| Autoři | |
|---|---|
| Rok publikování | 2016 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Journal of Proteomics |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://www.sciencedirect.com/science/article/pii/S1874391915301986?via%3Dihub |
| Doi | http://dx.doi.org/10.1016/j.jprot.2015.11.025 |
| Obor | Biochemie |
| Klíčová slova | Breast cancer; Lymph node metastasis; Transgelin; Transgelin-2; Proteomics |
| Popis | Transgelin and transgelin-2 have been discussed as potential markers of various cancers. Here we identified increased transgelin level in lymph node positive vs. negative, low grade primary breast cancer tissues using 2-DE in the cohort of 12 patients.Wefurther clinically validated 2-DE results in an independent cohort of 48 lowgrade breast cancer patients through untargeted and targeted proteomics analysis (iTRAQ-2D-LC–MS/MS, mTRAQSRM), at transcript level and using immunohistochemistry. Another group of 48 high grade tumors of different breast cancer subtypes was analyzed together with the low grade samples to test transgelin specificity for low grade tumors and to study transgelin relation to known molecular markers and histological features. The results confirmed transgelin connection with the lymph node metastasis. As a marker of a reactive tumor stroma, transgelin can be connected with the higher risk of metastasis development. Moreover, we observed significant down-regulation of transgelin in high vs. low grade tumors caused by decreased content of stromal cells (mainly expressing transgelin) in high grade tumor tissue. We also analyzed expression of transgelin-2 in the second cohort using proteomics and immunohistochemistry. Transgelin-2 was mainly expressed by epithelial cancer cells and its levels were increased in metastatic and poorly differentiated tumors. |
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