Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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VESELÝ Radek JELINKOVA Pavlina HEGEROVA Dagmar CERNEI Natalia KOPEL Pavel MOULICK Amitava RICHTERA Lukas HEGER Zbynek ADAM Vojtech ZITKA Ondrej

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Materials
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www http://www.mdpi.com/journal/materials
Doi http://dx.doi.org/10.3390/ma9040260
Obor Traumatologie a ortopedie
Klíčová slova branched chain amino acids; encapsulation; Escherichia coli; nanomedicine; Staphylococcus aureus
Popis This paper investigates the synthesis of paramagnetic nanoparticles, which are able to bind branched chain amino acids (BCAAs)-leucine, valine, and isoleucine and, thus, serve as a tool for their isolation. Further, by this, we present an approach for encapsulation of nanoparticles into a liposome cavity resulting in a delivery system. Analyses of valine and leucine in entire complex show that 31.3% and 32.6% recoveries are reached for those amino acids. Evaluation of results shows that the success rate of delivery in Escherichia coli (E. coli) is higher in the case of BCAAs on nanoparticles entrapped in liposomes (28.7% and 34.7% for valine and leucine, respectively) when compared to nanoparticles with no liposomal envelope (18.3% and 13.7% for valine and leucine, respectively). The nanoparticles with no liposomal envelope exhibit the negative zeta potential (-9.1 +/- 0.3 mV); however, their encapsulation results in a shift into positive values (range of 28.9 +/- 0.4 to 33.1 +/- 0.5 mV). Thus, electrostatic interactions with negatively-charged cell membranes (approx. -50 mV in the case of E. coli) leads to a better uptake of cargo. Our delivery system was finally tested with the leucine-rich antimicrobial peptide (FALALKALKKALKKLKKALKKAL) and it is shown that hemocompatibility (7.5%) and antimicrobial activity of the entire complex against E. coli, Staphylococcus aureus (S. aureus), and methicilin-resistant S. aureus (MRSA) is comparable or better than conventional penicillin antibiotics.
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