Azidopropylvinylsulfonamide as a New Bifunctional Click Reagent for Bioorthogonal Conjugations: Application for DNA-Protein Cross-Linking

• Autoři: DADOVÁ Jitka; VRÁBEL Milan; ADÁMIK Matej; BRÁZDOVÁ Marie; POHL Radek; FOJTA Miroslav; HOCEK Michal
• Rok publikování: 2015
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Chemistry - A European Journal
• Fakulta / Pracoviště MU: Středoevropský technologický institut
• www: http://onlinelibrary.wiley.com/doi/10.1002/chem.201502209/epdf
• Doi: http://dx.doi.org/10.1002/chem.201502209
• Obor: Biochemie
• Klíčová slova: biotransformations; click chemistry; conjugation; nucleic acids; proteins
• Popis: N-(3-Azidopropyl)vinylsulfonamide was developed as a new bifunctional bioconjugation reagent suitable for the cross-linking of biomolecules through copper(I)-catalyzed azide-alkyne cycloaddition and thiol Michael addition reactions under biorthogonal conditions. The reagent is easily clicked to an acetylene-containing DNA or protein and then reacts with cysteine-containing peptides or proteins to form covalent cross-links. Several examples of bioconjugations of ethynyl- or octadiynyl-modified DNA with peptides, p53 protein, or alkyne-modified human carbonic anhydrase with peptides are given.

Související projekty

• Centrum nových přístupů k bioanalýze a molekulární diagnostice