Isolation of human pathogen Escherichia albertii from faeces of seals (Leptonychos weddelli) in James Ross Island, Antarctica

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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SEDLÁČEK Ivo GRILLOVÁ Linda STAŇKOVÁ Eva ČERNOHLÁVKOVÁ Jitka ŠMAJS David

Rok publikování 2013
Druh Článek v odborném periodiku
Časopis / Zdroj Czech polar Reports
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Obor Mikrobiologie, virologie
Klíčová slova identification; bacteriocin; biotyping; ribotyping
Popis A set of nine gram-negative fermenting rods biochemically identified as Escherichia coli was isolated from faeces of seals. These bacteria were characterized by phenotypic classification, 16S rDNA sequence analyses, automated ribotyping, study of whole-cell protein profiles by SDS-PAGE and finally by bacteriocin production. The results of our polyphasic taxonomic study supported the recognition of P4652, P4653 and P4740 isolates as true members of Escherichia albertii species – probably a major enteric human pathogen. To our best knowledge, this is the first evidence showing that E. albertii produces bacteriocin, and colicin D. Obtained data unambiguously showed inconvenience of commercial identification systems to distinguish both Escherichia species due to missing data of E. albertii in the commercial databases. The results of Escherichia isolates taxonomy suggest seals as a novel source of human and animal pathogen, E. albertii in the Antarctic region.
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