“Omics” and population genetic tools applied on selected species from the class Monogenea
| Autoři | |
|---|---|
| Rok publikování | 2015 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Our study is focused on three model organisms Eudiplozoon nipponicum, Paradiplozoon homoion and Paradiplozoon gracile all of them are representing blood sucking fish ectoparasites from group Monogenea: Diplozoidae. Our project consists of two parts according to the used methods. The first part combining the modern pyro-sequencing methods and computer databases for the faster and more accurately identification of numerous sets of protein molecules, which are essential for life of monogeneans. The second part of study is more focused on population genetics and description of each sample at the individual level. In order to obtain this type of data, we decided to use amplified fragment length polymorphism technique (AFLP). Our four aims are realized; (i) compare the genetic diversity in populations of monogenean species, (ii) determine the level of genetic variability of the two permanently fused worms isolated from the same fish (“sex factor”), (iii) reveal the intra- and inter-population patterns and (iv) evaluate the effect of different host species on genetic plasticity of monogenea with generalist life strategy. We started the preliminary analyses leading to generation of E. nipponicum genome, transcriptome and proteome databases. We would like to use data platforms for identification and further characterization of protein molecules involved into the interaction between host and parasite. In the order to check the volume of obtained genomic data vs. reality we used the DNA fluorescent double staining method, which represents simple and easy checking point for obtained bioinformatics data. This method was designed for single-celled organisms and therefore we had to optimise the dying protocol and we are currently working on analysis settings for multi-celled organisms. The mRNA E. nipponicum adult in the form of sort reads/transcripts was analyzed using specific software tools. e.g. Trimmomatic, SPAdes, TopHat, Bowtie2, Trinity, SOAPdenovo and Velvet-Oases. |
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