N-(2-Hydroxypropyl) Methacrylamide Based Cryogels – Synthesis and Biomimetic Modification for Stem Cell Applications

Varování

Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	GOLUNOVA A. JAROŠ Josef JURTÍKOVÁ Veronika KOTELNIKOV I. KOTEK J. HLÍDKOVÁ H. STREIT Libor HAMPL Aleš RYPÁČEK F. PROKS V.
Rok publikování	2015
Druh	Článek v odborném periodiku
Časopis / Zdroj	Physiological Research
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
Obor	Genetika a molekulární biologie
Klíčová slova	Hydrogels; Cryogels; Stem cells; Scaffolds; Mechanical properties
Popis	The design of favorable mechanical properties and suitable surface modifications of hydrogels in order to stimulate specific cell response is a great challenge. N-(2-Hydroxypropyl) methacryl-amide (HPMA) was utilized to form macroporous cryogel scaffolds for stem cell applications. Furthermore, one group of scaffolds was enhanced by copolymerization of HPMA with methacryloyl-GGGRGDS-OH peptide in an effort to integrate biomimetic adhesion sites. The cryogels were characterized by stiffness and equilibrium swelling measurements as well as by scanning electron microscopy. Cell culture experiments were performed with human adipose-derived stem cells and substrates were found completely non-toxic. Moreover, RGDS-enriched cryogels supported cell attachment, spreading and proliferation, so they can be considered suitable for designed aims.
Související projekty:	Centrum analýz a modelování tkání a orgánů Zdroje pro tkáňové inženýrství 5