Rad51 Paralogs Remodel Pre-synaptic Rad51 Filaments to Stimulate Homologous Recombination

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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TAYLOR Martin R. G. ŠPÍREK Mário CHAURASIYA Kathy R. WARD Jordan D. CARZANIGA Raffaella YU Xiong EGELMAN Edward H. COLLINSON Lucy M. RUEDA David KREJČÍ Lumír BOULTON Simon J.

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Cell
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1016/j.cell.2015.06.015
Obor Genetika a molekulární biologie
Klíčová slova STRAND BREAK REPAIR; CANCER SUSCEPTIBILITY GENE; RECA PROTEIN; FANCONI-ANEMIA; DNA-DAMAGE; SACCHAROMYCES-CEREVISIAE; GERMLINE MUTATIONS; OVARIAN-CANCER; BRCA2; PROMOTES
Popis Repair of DNA double strand breaks by homologous recombination (HR) is initiated by Rad51 filament nucleation on single-stranded DNA (ssDNA), which catalyzes strand exchange with homologous duplex DNA. BRCA2 and the Rad51 paralogs are tumor suppressors and critical mediators of Rad51. To gain insight into Rad51 paralog function, we investigated a heterodimeric Rad51 paralog complex, RFS-1/RIP-1, and uncovered the molecular basis by which Rad51 paralogs promote HR. Unlike BRCA2, which nucleates RAD-51-ssDNA filaments, RFS-1/RIP-1 binds and remodels pre-synaptic filaments to a stabilized, "open,'' and flexible conformation, in which the ssDNA is more accessible to nuclease digestion and RAD-51 dissociation rate is reduced. Walker box mutations in RFS-1, which abolish filament remodeling, fail to stimulate RAD-51 strand exchange activity, demonstrating that remodeling is essential for RFS-1/RIP-1 function. We propose that Rad51 paralogs stimulate HR by remodeling the Rad51 filament, priming it for strand exchange with the template duplex.
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