Transcriptomic analyses of a monogenean: Next-Generation Sequencing techniques in hands of parasitologists.

Logo poskytovatele
Logo poskytovatele

Varování

Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
Autoři

DVOŘÁKOVÁ Hana JEDLIČKOVÁ Lucie KAŠNÝ Martin BROŽ Petr ILGOVÁ Jana GELNAR Milan MIKEŠ Libor

Rok publikování 2015
Druh Konferenční abstrakty
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Popis Next-generation sequencing methods currently represent a robust tool enabling significant enrichment of our knowledge of biological systems at a level which has not been possible ever before. Unfortunately, the transcriptomic/genomic data on members of Monogenea are still extremely limited. Therefore we adopted the NGS techniques to generate a first high quality transcriptome of a representative of blood-feeding monogeneans - Eudiplozoon nipponicum (Diplozoidae: Heteronchoinea), the ectoparasite from the gills of common carp Cyprinus carpio. Using 454 sequencing technology (GS FLX System, Roche), a total of 324 941 reads were generated (average length 423 bp) and assembled into 6910 contigs. The second transcriptome analysis was performed by the MiSeq System (Illumina). A total of 74 823 255 input read pairs, a total of 142 813 all transcripts (median contig length 367 bp) and a total of 119 136 of genes (median contig length 334 bp) were obtained. Currently, the comprehensive bioinformatic analysis is running - the assemblies are in the process of evaluation and a pipeline for annotation of particular genes is being developed. Special attention was paid to peptidases that could be involved in digestion of host´s blood and other biological processes in E. nipponicum.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.