Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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PELCOVÁ Marta LANGMAJEROVÁ Monika CVINGRÁFOVÁ Eliška JUŘICA Jan GLATZ Zdeněk

Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj JOURNAL OF SEPARATION SCIENCE
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Doi http://dx.doi.org/10.1002/jssc.201400582
Obor Biochemie
Klíčová slova Dextromethorphan; Dextrorphan; Methanol; Nonaqueous media
Popis This study deals with the nonaqueous capillary electrophoretic separation of dextromethorphan and its metabolites using a methanolic background electrolyte. The optimization of separation conditions was performed in terms of the resolution of dextromethorphan and dextrorphan and the effect of separation temperature, voltage, and the characteristics of the background electrolyte were studied. Complete separation of all analytes was achieved in 40 mM ammonium acetate dissolved in methanol. Hydrodynamic injection was performed at 3 kPa for 4 s. The separation voltage was 20 kV accompanied by a low electric current. The ultraviolet detection was performed at 214 nm, the temperature of the capillary was 25°C. These conditions enabled the separation of four analytes plus the internal standard within 9 min. Further, the developed method was validated in terms of linearity, sensitivity, and repeatability. Rat liver perfusate samples were subjected to the nonaqueous capillary electrophoretic method to illustrate its applicability.
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