Improved IgH-based MRD Detection By Using Droplet Digital PCR: a Comparison With Real Time Quantitative PCR in MCL and MM
| Autoři | |
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| Rok publikování | 2013 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Minimal residual disease (MRD) detection is a well defined prognostic tool for response evaluation in hematological malignancies. Real time quantitative PCR (RQ-PCR) has become the settled and standardized method for MRD assessment in lymphoid disorders (van der Velden et al., Leukemia 2007). However, RQ-PCR based MRD detection relies on a relative quantification approach, since it requires a reference standard curve and an appropriate housekeeping gene for the normalization. Droplet Digital PCR (DD-PCR), applying the principle of limiting dilution of DNA and single molecule detection, allows reliable absolute tumor burden quantification at molecular level. In this study we performed a comparison of immunoglobulin heavy chain gene based MRD detection (IgH-MRD) performed by RQ-PCR and DD-PCR, to assess whether DD-PCR could overcome some RQ-PCR limitation and achieve superior sensitivity, robustness and accuracy. |
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