POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS
| Autoři | |
|---|---|
| Rok publikování | 2012 |
| Druh | Článek ve sborníku |
| Konference | CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Analytická chemie, separace |
| Klíčová slova | Exhaled breath condensate; capillary electrophoresis; point of care diagnostics |
| Popis | Exhaled breath condensate has been analyzed for its ionic content by capillary electrophoresis with capacitively coupled contactless conductometric detection. Two devices for exhaled breath condensate collection were compared. These include a tube-in-tube cooled sampler and a simple zip-lock bag. The devices allow collection of small volumes (100-200 mu L) of exhaled breath condensate in less than 2 min. A method for quick (less than 3 min) simultaneous determination of inorganic cations, inorganic anions and organic anions from the samples using dual-opposite end injection principle with a short fused silica capillary (35 cm, 50 mu m i.d.) was optimized with final background electrolyte composition of 20 mM 2-(N-morpholino) ethanesulfonic acid, 20 mM L-histidine, 30 mu M cetyltrimethylammonium bromide and 2 mM 18-crown-6 was used. It has been shown that changes of nitrite could be observed in acute inflammation of upper airways and in person with diagnosed mild chronic obstructive pulmonary disease, while the changes of other ions could also be observed. |
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