CAPILLARY ELECTROPHORETIC SCREENING OF TOXIC METABOLITES IN VARIOUS BODY FLUIDS - A SIMPLE DIAGNOSTIC TOOL TO DIFFERENTIATE METHANOL AND ETHYLENE GLYCOL POISONING

• Autoři: ĎURČ Pavol; KUBÁŇ Petr; BITTOVÁ Miroslava; FORET František
• Rok publikování: 2013
• Druh: Článek ve sborníku
• Konference: Chemické listy
• Fakulta / Pracoviště MU: Středoevropský technologický institut
• www: Chemické listy
• Obor: Analytická chemie, separace
• Klíčová slova: capillary electrophoresis; contactless conductivity detection; methanol; ethylenglycol; toxic metabolites
• Popis: A new, simple and rapid capillary electrophoretic method with contactless conductivity detection for screening of formate, oxalate and glycolate in various body samples (blood serum, saliva, urine, exhaled breath condensate) is presented. The target analytes are separated in less than 6 minutes in an electrolyte composed of 50 mM L-histidine and 50 mM 2-(N-morpholino) ethanesulfonic acid at pH 5.9. A short (33 cm) fused silica capillary with 25 um ID is used. LODs range from 0.4 to 1.25 uM. The method provides a simple and rapid diagnostic test in suspected intoxication of industrial chemicals and is able to distinguish the ingested liquid, based on its metabolite trace providing a fast screening tool that can be applicable in clinical practice.

Související projekty

• CEITEC - středoevropský technologický institut
• Vývoj neinvazivních metod odberu vzorku a analýzy v point-of-care dignostice biologických materiálu