DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin.
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Rok publikování | 2013 |
Druh | Článek v odborném periodiku |
Časopis / Zdroj | Biochimie |
Fakulta / Pracoviště MU | |
Citace | |
Doi | http://dx.doi.org/10.1016/j.biochi.2013.07.025 |
Obor | Biochemie |
Klíčová slova | Haloalkane Dehalogenase |
Popis | Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards beta-brominated alkanes. Moreover, we identified other putative dehalogenases of eukaryotic origin, representing targets for future experiments to discover dehalogenases with novel catalytic properties. |
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