Strong Accumulation of Chloroplast DNA in the Y Chromosomes of Rumex acetosa and Silene latifolia

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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ŠTEFLOVÁ Pavlína HOBZA Roman VYSKOT Boris KEJNOVSKÝ Eduard

Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj Cytogenetic and Genome Research
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://www.karger.com/Article/FullText/355212
Doi http://dx.doi.org/10.1159/000355212
Obor Genetika a molekulární biologie
Klíčová slova Chloroplast DNA; Rumex acetosa; Sex chromosomes; Silene latifolia
Popis Chloroplast DNA (cpDNA) sequences are often found in plant nuclear genomes, but patterns of their chromosomal distribution are not fully understood. The distribution of cpDNA on the sex chromosomes can only be studied in dioecious plant species possessing heteromorphic sex chromosomes. We reconstructed the whole chloroplast genome of Rumex acetosa (sorrel, XY1Y2 system) from next generation sequencing data. We systematically mapped the chromosomal localization of various regions of cpDNA in R. acetosa and in Silene latifolia (white campion, XY system) using fluorescence in situ hybridization. We found that cpDNA was accumulated on the Y chromosomes of both studied species. In R. acetosa, the entire Y chromosome gathered all parts of cpDNA equally. On the contrary, in S. latifolia, the majority of the cpDNA, corresponding to the single copy regions, was localized in the centromere of the Y chromosome, while the inverted repeat region was present also in other loci. We found a stronger accumulation of cpDNA on the more degenerated Y1 and Y2 chromosomes of R. acetosa than in evolutionary younger S. latifolia Y chromosome. Our data stressed the prominent role of the Y chromosome centromere in cpDNA accumulation.
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