Capillary electrophoresis with contactless conductometric detection for rapid screening of formate in blood serum after methanol intoxication
| Autoři | |
|---|---|
| Rok publikování | 2013 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Journal of Chromatography A |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://www.ncbi.nlm.nih.gov/pubmed/23419511 |
| Doi | http://dx.doi.org/10.1016/j.chroma.2013.01.035 |
| Obor | Analytická chemie, separace |
| Klíčová slova | Capillary electrophoresis; Contactless conductivity detection; Formate; Methanol intoxication |
| Popis | A new method for rapid, direct determination of formate in blood serum samples by capillary electrophoresis with contactless conductometric detection is presented. A selective separation of formate was achieved in approximately 1 min using an electrolyte system comprising 10 mM L-histidine, 15 mM glutamic acid and 30 mu M cetyltrimethylammonium bromide at pH 4.56. The only sample preparation was dilution (1:100) with deionized water. The limit of detection and limit of quantitation was 2.2 mu M and 7.3 mu M, respectively, which corresponds to 0.22 mM and 0.73 mM in undiluted blood serum. The method provides a simple and rapid diagnostic test in suspected methanol intoxication cases. The method has been successfully tested on determination of formate in blood of a patient admitted to the hospital under acute methanol intoxication. The peak concentration of formate detected in the patient blood serum was 12.4 mM, which is 10- to 100-fold higher than the normal values in healthy population. The developed method presents the fastest test currently available to detect formate in blood samples. |
| Související projekty: |