Informace o projektu
Analysis of gastrointestinal microbiome in patients with primary immune deficiencies
- Kód projektu
- MUNI/M/1322/2015
- Období řešení
- 3/2016 - 12/2018
- Investor / Programový rámec / typ projektu
-
Masarykova univerzita
- Grantová agentura MU
- INTERDISCIPLINARY - Mezioborové výzkumné projekty
- Fakulta / Pracoviště MU
- Lékařská fakulta
- Další fakulta/pracoviště MU
- Fakulta informatiky
- Další fakulta/pracoviště MU
- Středoevropský technologický institut
In the past decade, gut microbiota has been shown to be associated with a number of human diseases including metabolic syndrome, obesity-related diseases, inflammatory bowel disease, and colorectal cancer. In this project, we plan to study human microbiome in primary antibody immune deficiencies, common variable immunodeficiency (CVID) and selective IgA deficiency (IgAD). In both diseases, IgA production is seriously affected. Since IgA is the dominant mucosal immunoglobulin and plays a central role in maintaining intestinal homeostasis, it is expected that i) the gut microbiome reflects changes in host mucosal immune system. In addition, since there is a significant portion of patients without clinical symptoms or with very mild symptoms, it is likely that ii) the microbiome composition is of importance in these inapparent disease cases. To determine microbiome composition, 16S rRNA gene sequences from the gut microbiome will be examined in all DNA samples (n =50) isolated from patients and healthy controls. In addition, analysis of gut metagenome by whole-metagenome shotgun sequencing and subsequent analysis of gut metabolome will be performed in 6-10 samples previously characterized by the analysis of 16S rRNA gene sequences. This will allow characterization of microbial communities present in the samples and determination of alpha and beta diversity of the samples. In addition, functional characterization of human microbiome will be performed together with determination of the host’s metabolome in a number of selected samples.
Publikace
Počet publikací: 11
2021
-
Patients With Common Variable Immunodeficiency (CVID) Show Higher Gut Bacterial Diversity and Levels of Low-Abundance Genes Than the Healthy Housemates
Frontiers in Immunology, rok: 2021, ročník: 12, vydání: May 2021, DOI
2020
-
Administration of the Probiotic Escherichia coli Strain A0 34/86 Resulted in a Stable Colonization of the Human Intestine During the First Year of Life
Probiotics and Antimicrobial Proteins, rok: 2020, ročník: 12, vydání: 2, DOI
2019
-
Bacterial and fungal gut microbiota analysis in common variable immunodeficiency (CVID) disorders: paired case-control study
Rok: 2019, druh: Konferenční abstrakty
-
Bacterial but Not Fungal Gut Microbiota Alterations Are Associated With Common Variable Immunodeficiency (CVID) Phenotype
Frontiers in Immunology, rok: 2019, ročník: 10, vydání: 1914, DOI
-
Gut microbiome analysis in common variable immunodeficiency (CVID) patients and paired healthy controls sharing the same household
Rok: 2019, druh: Konferenční abstrakty
-
The Impact of DNA Extraction Methods on Stool Bacterial and Fungal Microbiota Community Recovery
Frontiers in Microbiology, rok: 2019, ročník: 10, vydání: 821, DOI
2018
-
Analýza mykobiomu aneb chybami se člověk učí.
Rok: 2018, druh: Konferenční abstrakty
-
Artificial fungal community (fMOCK) as a control of sequencing process in mycobiome research.
Rok: 2018, druh: Konferenční abstrakty
-
Gut microbiome analysis in common variable immunodeficiency (CVID) patients.
Rok: 2018, druh: Konferenční abstrakty
2017
-
Comparison of five bacterial DNA extraction methods from germ-free mice fecal samples
Rok: 2017, druh: Konferenční abstrakty