The Use of Magnetic poly(N-isopropylacrylamide) Microspheres for Separation of DNA from Probiotic Dairy Products

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Authors	MACKOVÁ Hana HORÁK Daniel TRACHTOVÁ Štěpánka RITTICH Bohuslav ŠPANOVÁ Alena
Year of publication	2012
Type	Article in Periodical
Magazine / Source	Journal of Colloidal Science and Biotechnology
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Description	Magnetic thermosensitive poly(N-isopropylacrylamide) (PNIPAAm) microspheres 3.6 mikrom in size were prepared by the inverse emulsion polymerization in paraffin oil in the presence of maghemite (gama-Fe2O3) nanoparticles. Gama-Fe2O3 nanoparticles (11 nm) were obtained by coprecipitation of Fe(II) and Fe(III) chlorides in a basic solution followed by oxidation with NaClO. The size, polydispersity and iron content of the magnetic PNIPAAm microspheres were characterized by light microscopy, transmission electron microscopy (TEM) and atomic absorption spectroscopy (AAS). Thermosensitivity of water-swollen magnetic PNIPAAm microparticles was investigated by light microscopy equipped with a heating stage. Magnetic PNIPAAm microspheres were used for separation of DNA from probiotic dairy products (yoghurt) at different temperatures.
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