Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-g-lycidyl methacrylate) microspheres for selective isolation of bacterial DNA.

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Authors	HORÁK Daniel ŠPANOVÁ Alena TVRDÍKOVÁ Jana RITTICH Bohuslav
Year of publication	2011
Type	Article in Periodical
Magazine / Source	European Polymer Journal
MU Faculty or unit	Faculty of Science
Citation
Doi	http://dx.doi.org/10.1016/j.eurpolymj.2011.02.007
Field	Macromolecular chemistry
Keywords	Glycidyl methacrylate; 2-hydroxyethyl methacrylate; Streptavidin; Biotin; Magnetic Microspheres; DNA
Description	The dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) with glycidyl methacrylate (GMA) in a toluene/2-methylpropan-1-ol mixture in the presence of Fe3O4 nanoparticles coated with oleic acid, produced monodisperse magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-GMA)) microspheres. Oxirane groups of the microspheres were ammonolyzed and then functionalized with streptavidin using cyanuric chloride. The final product contained 0.67 mg of streptavidin per gram of wet magnetic P(HEMA-GMA) microspheres. The microspheres were characterized by elemental analysis, scanning electron microscopy, IR, UV–VIS and atomic absorption spectroscopy The streptavidin-modified magnetic P(HEMA-GMA) microspheres were used for immobilization of biotinylated DNA and subsequent selective isolation of target DNA from complex samples using DNA/DNA hybridisation.
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