Selective isolation of DNA from food matrices
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Proceedings |
| Conference | 30th International Symposium on the Separation of Proteins, Peptides and Polynucleotides |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | magnetic particles; streptavidin; Lactobacillus DNA separation; PCR |
| Description | Research has been focused on the development of innovative magnetic micrometer-sized microspheres for specific applications in DNA analysis in food matrices and other real samples. Selective isolation of high-quality DNA can be performed using suitable functionalised magnetic microspheres. Magnetic P(HEMA-co-GMA) microspheres functionalised with streptavidin were used for immobilisation of biotinylated DNA probes (primers, PCR products) and for selective DNA isolation of Lactobacillus paracasei using DNA/DNA hybridisation. The procedure proposed was applied for target isolation of DNA from probiotic bacteria in complex samples (probiotic food supplements). Isolated DNA was used in genus- and species-specific PCRs. |
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